Screen and filter antibodies and sera. Bio-Dot, from Bio-Rad laboratories, is a simple device that offers a reproducible method for binding proteins or nucleic acids onto nitrocellulose or Zeta-Probe membrane. ZERO BIAS - scores, article reviews, protocol conditions and more. Screen and filter antibodies and sera. Leak-free, modular, and autoclavable template. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle,. Microfiltration (Dot Blotting) Blotting Systems and Power Supplies 12 Tank Blotting Cells6312 ®Mini Trans-Blot Cell and Criterion ™ Blotter 12 ®Trans-Blot Cell12 Immun-Blot ®Trans-Blot Plus Cell13 Semi-Dry Blotting Cells 13 Trans-Blot® SD Semi-Dry Cell and Detection Kits14 ™Trans-Blot® Turbo System14Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle,. Leak-free, modular, and autoclavable template. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. In short, total protein (25 μl per well of a 96 well plate) was transferred onto a nitrocellulose membrane, blocked for 2 h with 5% nonfat milk in TBST (Tris-buffered saline, 0. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Screen and filter antibodies and sera. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. Screen and filter antibodies and sera. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. The Bio-Dot apparatus is provided as a complete unit, or as a modular. The Bio-Dot microfiltration apparatus can be used for any application requiring rapid immobilization and screening of unfractionated or purified proteins, nucleic acids, or. List Price: Your Price: Log in to see your price. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. The Bio-Dot Microfiltration System is available as a complete standalone unit (170-3938) or as a modular template without the manifold base ( 170-6547 ). Unit does not include membrane, gasket support plate, or other consumables. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Bioz Stars score: 96/100, based on 1 PubMed citations. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. 1 Autoclaving The Tygon. Features of the Bio-Dot SF Microfiltration System:Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Prezzo di listino: Your Price: Log in to see your price. As shown in figure 1, a sheet of membrane is clamped between the gasket and the 96-well sample template. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. 5 mg/g of vitamin K1,AB diets,Code 4165. Screen and filter antibodies and sera. The Bio-Dot apparatus is provided as a complete unit, or as a modular addition to the Bio-Dot SF slot format microfiltration apparatus. BIO-RAD Bio-Dot Microfiltratio n Apparatus 84BR 96-well #170-6545 NEW Be the first to write a review. Screen and filter antibodies and sera. Many experimental protocols can be accommodated by using interchangeable templates. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Unit does not include membrane, gasket support plate, or other consumables. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Screen and filter antibodies and sera. . Vortex (Fisher scientific, model: ZX Wizard vortex, catalog number: 11746744) 12. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. The Bio-dot® Microfiltration Apparatus is in a 96 well-plate format, allowing analysis of many small samples at once. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. Leak-free, modular, and autoclavable template. The membrane with bound antigen is then incubated with first antibody, specific for the antigen to be detected. Bioz Stars score: 96/100, based on 1 PubMed citations. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Many experimental protocols can be accommodated by using interchangeable templates. Description. Buffer solutions move in uniform capillary action bringing proteins clearly to surface for further sequence. Quantità: Aggiungi a carrello. Part II – Slot Blotting 1. The 96-well Bio-Dot and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Vacuum pump 10. Leak-free, modular, and autoclavable template. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. Leak-free, modular, and autoclavable template. Use the Bio-Dot SF Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 48-well slot format. Leak-free, modular, and autoclavable template. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Reproducibly capture proteins or core acids in solution onto membranes with Bio-Dot microfiltration systems. This instrument allows rapid, reproducible applications of up to 96 samples on one membrane sheet. The 96-well Bio-Dot and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Rehydrate membrane with ~200ml 10X SSC per well. Welcome to my auction! VINTAGE BIO RAD BIO DOT 1706545 96 WELL MICROFILTRATION BLOTTING LAB APPARATUS! We are downsizing items from our laboratories in order to raise research money for our exciting project on brain trauma. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Last modified: July 8, 2003 MATERIALS: DRY Bio-Rad Bio-Dot SF Microfiltration Apparatus Nytran membrane Bio-Dot SF filter paper 60 3mm Whatman paper. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Screen and filter antibodies and sera. Leak-free, modular, and autoclavable template. Reproducibly capture proteins or nucleus acids in solution onto membranes with Bio-Dot microfiltration solutions. Screen and filter antibodies and sera. S. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Table of Contents Page. This slot format makes it. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. com today. Section 3 Dot Blotting. The 96-well Bio-Dot and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Web site USA 800 4BIORAD Australia 02 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 21 3237 9400 Canada 905 712 2771 China 86 21 6426 0808 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00 France 01 47 95 69 65. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketScreening of membrane proteins solubilization. Precio de lista: Your Price: Log in to see your price. leakage, whether the vacuum is on or off. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketMicrofiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasket Bio-Dot Apparatus #1706545 | Bio-Rad Skip to main contentBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Autoclaving 15 minutes at 250°F (121°C) with a 1 minute fast exhaust. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasket Bio-Dot Apparatus #1706545 | Bio-Rad Skip to main contentReproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. The Bio-Dot SF Microfiltration System is available as. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Stock # 72926-1 . A modified 96-well dot-blot apparatus (Bio-Rad Laboratories Inc. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketReproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. PAGE 6 Section 2 Special Handling Features The Bio-Dot apparatus withstands autoclave temperatures for sterilization, as well as cleaning with alcohols, acids, and base solutions. The system is designed to sandwich a membrane between a plastic frame and rubber gasket with 96 holes (below the membrane) and a plastic frame with 96 open-bottom. As the sample is concentrated in a small area, this format also allows easy quantification of samples using a densitometer or the Odyssey Imaging system. Leak-free, modular, and autoclavable template. Leak-free, modular, and autoclavable template. Leak-free, modular, and autoclavable template. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Bio-Rad 96-Well Bio-Dot Microfiltratio n Apparatus, 1706545, Blotting DeviceMicro. Leak-free, modular, and autoclavable template. Screen and filter antibodies and sera. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasket Bio-Dot Apparatus #1706545 | Bio-Rad Skip to main content Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. 1. 40–60 µg of secretome proteins in 100 µL PBS were dot blotted onto nitrocellulose membranes using the Bio-Dot 96-Well Microfiltration. Screen and filter antibodies and sera. ZERO BIAS - scores, article reviews, protocol conditions and more. Many experimental protocols can be accommodated by using interchangeable templates. Leak-free, modular, and autoclavable template. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. 1 Autoclaving The Tygon. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasket8. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Use the Bio-Dot SF Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 48-well slot format. 0. Screen and filter antibodies and sera. Screen and filter antibodies and sera. Leak-free, modular, and autoclavable template. Price from $9. Slot blotting microfiltration device, includes 48-well slot-format sample template, vacuum manifold base, gasket support plate, gasket, filter paper Product Specs; Company Info;. 75 mm. (b). Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketThe two most common methods for protein transfer are: Electrophoretic transfer — proteins separated in gels (for example, following polyacrylamide gel electrophoresis, or PAGE), are transferred onto membranes by electrophoretic transfer; Microfiltration (dot blotting) — proteins in solution are bound to membranes by vacuum driven microfiltration. Screen and filter antibodies and sera. Many experimental protocols can be accommodated by using interchangeable templates. 2. La lista activa. Please search here. Leak-free, modular, and autoclavable template. Description. Bio rad dot blot apparatus manualidades Bio-Rad 96 well dot blot apparatus 96 Well Dot Blot Apparatus, supplied by Bio-Rad, used in various techniques. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketConversion of the Bio-Dot apparatus to the Bio-Dot SF apparatus is accomplished by purchasing the Bio-Dot SF module, which provides the 48-well slot format sample template. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Many experimental protocols can be accommodated by using interchangeable templates. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Screen and filter antibodies and sera. Description. Leak-free, modular, and autoclavable template. , call 1-800-424-6723. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Screen and filter antibodies and sera. Leak-free, modular, and autoclavable template. Features of the Bio-Dot Microfiltration System: Resistance to 100% ethanol, strong acid, and NaOH; Autoclavability; Patented sealing gasket to eliminate lateral leakagePlace a pre-wetted membrane in the 96-well Bio-Dot® Microfiltration Apparatus (Bio-Rad) according to the manufacturer’s instructions. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. This Bio-Rad Bio-Dot Apparatus is used and in excellent condition. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Conversion of the Bio-Dot SF apparatus to the Bio-Dot blotting apparatus is accomplished by purchasing the Bio-Dot module, which provides the 96-well sample template. Many experimental protocols can be accommodated by using interchangeable templates. Many experimental protocols can be accommodated by using interchangeable templates. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. Leak-free, modular, and autoclavable template. Leak-free, modular, and autoclavable template. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Meeting Your Blotting Needs Turn to Bio-Rad for: • Gel-blotting equipment for an array of gel sizes • Microfiltration devicesMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasket96 Well Vacuum Dot Blotting Apparatus, supplied by Bio-Rad, used in various techniques. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. Many experimental protocols can be accommodated by using interchangeable templates. Leak-free, modular, and autoclavable template. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. A microfiltration apparatus was used to define wells over each sample detection region (Bio-Dot ® Microfiltration apparatus from Bio-Rad, Hercules, CA, USA). Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Screen and filter antibodies and sera. Chemical compatibility The Bio-Dot SF apparatus can be used with 100% alcohol solutions. , 1997;. when a semi dry apparatus is used with a well optimized gel and transfer system. Leak-free, modular, and autoclavable template. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Bio-Rad Laboratories, Inc. 1 watched in the last 24 hours. Screen and filter antibodies and sera. 12 4006127. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Screen and filter antibodies and sera. Screen and filter antibodies and sera. Measure [protein] by Lowry method using Bio-Rad DC Protein Assay. Leak-free, modular, and autoclavable template. Connect the vacuum line and leave it open to the atmosphere during sample application. Bio-Dot® and. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Reproducibly acquire proteins or nucleic sours in solution for membranes with Bio-Dot microfiltration systems. The gasket is aligned Bio-Dot microfiltration system. Bioz Stars score: 96/100, based on 1 PubMed citations. Leak-free, modular, and autoclavable template. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Features of the Bio-Dot Microfiltration System: Resistance to 100% ethanol, strong acid, and NaOH; Autoclavability; Patented sealing gasket to eliminate lateral leakageReproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Leak-free, modular, and autoclavable template. S. Features of the Bio-Dot Microfiltration System: Resistance to 100% ethanol, strong acid, and NaOH; Autoclavability; Patented sealing gasket to eliminate lateral leakage Overview. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. Screen and filter antibodies and sera. RNA BIO-DOT (‘SLOT BLOT’) Adapted from existing protocols by Vinh Pham. The 96-well Bio-Dot and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. ZERO BIAS - scores, article reviews, protocol conditions and moreMicrofiltration blotting— the Immun-Blot assay can easily be adapted for use in the Bio-Dot ® and Bio-Dot SF apparatus. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Screen and filter antibodies and sera. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Samples were placed in a 96-well plate with 20 µl 1 M NaOH/25 mM EDTA per well at 95 ℃ for 10 min by Bio-Rad Thermal cycle and put on ice immediately. 96ウェルのバイオドット、48ウェルのバイオドットSF （Slot Format） は溶液状態のタンパク質または核酸を簡便に再現性よくメンブレンに吸着させる装置です。. Leak-free, modular, and autoclavable template. Description. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Bioz Stars score: 93/100, based on 1 PubMed citations. 00. Leak-free, modular, and autoclavable template. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketOverview. S. Leak-free, modular, and autoclavable template. Leak-free, modular, and autoclavable template. Bio-Dot has 96 wells that create 96 spots on the nitrocellulose. 170-6543. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. The wells are arranged in 8 rows and 6 columns. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. To examine expression of stress proteins, dot-blot assays were performed in a 96-well plate format using a Bio-dot microfiltration manifold (Bio-Rad, Hercules, CA). Leak-free, modular, and autoclavable template. Leak-free, modular, and autoclavable template. Bioz Stars score: 86/100, based on 1 PubMed citations. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Order Number: 1703938In brief, dot blots of genomic DNA and modified control oligonucleotides (unmethylated: oligo control, 5-methylated CpG: oligo 5mC, and 5-hydroxymethylated CpG: oligo 5hmC) were produced with Bio-Rad’s Bio-Dot Microfiltration apparatus according to manufacturer’s instructions. It cannot be used with. Web site USA 800 4BIORAD Australia 02 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 21 3237 9400 Canada 905 712 2771 China 86 21 6426 0808 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00 France 01 47 95 69 65. Many experimental protocols can be accommodated by using interchangeable templates. The well Bio-Dot ® and well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Unit does not include membrane, gasket support plate, or other. Screen and filter antibodies and sera. Many experimental protocols can be accommodated by using interchangeable templates. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketBio-Dot® SF Microfiltration Apparatus Instruction Manual, Rev DAfter 40 h of cultivation, cells were precipitated by centrifugation, and 0. Sample can be easily applied to the 96-well formate with a standard pipet or with the Costar. Screen and filter antibodies and sera. Leak-free, modular, and autoclavable template. Screen and filter antibodies and sera. Leak-free, modular, and autoclavable template. The Bio-Dot Microfiltration System is available as a complete standalone unit (170-3938) or as a modular template without the manifold base ( 170-6547 ). The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle,. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. Adapters are available for the application of either 96 “dots” in a 12x8 array, or as rectangular “slots” in a 6x8 array. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketOverview. Leak-free, modular, and autoclavable template. Leak-free, modular, and autoclavable template. With the Mini-PROTEAN II multiscreen apparatus, you can quickly and efficiently screen or filter up to 40 different antibodies or sera without cutting a western blot. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Bioz Stars score: 93/100, based on 1 PubMed citations. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasket Bio-Dot Apparatus #1706545 | Bio-Rad Skip to main content The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Many experimental protocols can be accommodated by using interchangeable templates. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. 1% Tween 20), probed with an anti-CR2 primary antibody. Description. Screen and filter antibodies and sera. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. The wells are arranged in 8 rows and 6 columns. 7. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. With the Mini-PROTEAN II multiscreen apparatus, you can quickly and efficiently screen or filter up to 40 different antibodies or sera without cutting a western. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Bio-Rad Bio-Dot 96-Well Microfiltration This Bio-Rad Bio-Dot Apparatus is used and in excellent condition. Depending of the intensity of the signal, solubilized fraction may be diluted prior to loading on the nitrocellulose membrane. Place a pre-wetted membrane in the 96-well Bio. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Screen and filter antibodies and sera. Sample can be easily applied to the 96-well formate with a standard pipet or with the Costar Octapette pipet. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Features of the Bio-Dot Microfiltration System: Resistance to 100% ethanol, strong acid, and NaOH; Autoclavability; Patented sealing gasket to eliminate lateral leakageReproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Screen and filter antibodies and sera. Title: 4006127. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Many experimental protocols can be accommodated by using interchangeable templates. Get a quote for a Used Bio Rad Bio Dot 96 Miscellaneous Test Equipment from DougDeals at used-line. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Condition: New Quantity: 3 available Price: US $499. Leak-free, modular, and autoclavable template. The 96-well Bio-Dot and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Fo r t e c h n i c a l s e r v i c e c a l l yo u r l o c a l B i o - R a d o f f i c e o r i n t h e U. Description. Use the Bio-Dot SF Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 48-well slot format. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. The Bio-Dot microfiltration apparatus can be used for any application requiring rapid immobilization and screening of unfractionated or purified proteins, nucleic acids, or macromolecular complexes on membranes, such as nitrocellulose or Zeta-Probe ® membrane. 00 $45. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Supernatants were collected, and interleukins (IL-1β. Configuration: • Sample Template • Sealing Gasket • Vacuum Manifold • Tubing and Flow Valve The Bio-dot® Microfiltration Apparatus is in a 96 well-plate format, allowing analysis of many small samples at once. Screen and filter antibodies and sera. Conversion of the Bio-Dot SF apparatus to the Bio-Dot blotting apparatus is accomplished by purchasing the Bio-Dot module, which provides the 96-well sample template. the kit) is used as a blocker when the Bio-Dot ® or Bio-Dot SF apparatus is used for blotting, since gelatin will obstruct filtration through blotting membranes. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Screen and filter antibodies and sera. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Leak-free, modular, and autoclavable template. Bio-Rad 96 well dot blot apparatus 96 Well Dot Blot Apparatus, supplied by Bio-Rad, used in various techniques. Cantidad: Añadir al carro. Phosphate-buffered saline (1× PBS): 25 mM Na 2 HPO 4, 150 mM NaCl, pH 8. The method described here uses the slot format (SF) of the Bio-Dot microfiltration apparatus (Bio-Rad). Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. The Bio-Dot apparatus is simple to operate. 1. Many experimental protocols can be accommodated by using interchangeable templates. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Screen and filter antibodies and sera. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasket. , c a l l 1 - 8 0 0 - 4 B I O R A D ( 1 - 8 0 0 - 4 2 4 - 6 7 2 3 ) Table of Contents. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Use the Bio-Dot Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 96-well format. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Both sample templates are interchangeable and use the same microfiltration manifold. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. The membrane should be removed from the apparatus after antigen application. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Leak-free, modular, and autoclavable template. 99 to $1999. The Bio-Dot SF apparatus focuses sample into a thin line instead of a circle for reproducible quantitation by densitometry. To examine expression of stress proteins, dot-blot assays were performed in a 96-well plate format using a Bio-dot microfiltration manifold (Bio-Rad, Hercules, CA). Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Leak-free, modular, and autoclavable template. For Dot Blotting, lysates containing equivalent amount of total protein were loaded on a 96-well plate (Bio-Dot ® Microfiltration Apparatus; Bio-Rad Laboratories Inc. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems. Many experimental protocols can be accommodated by using interchangeable templates. Leak-free, modular, and autoclavable template. Apply vacuum/atmosphere pressure. The 96-well Bio-Dot and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Features of the Bio-Dot Microfiltration System: Resistance to 100% ethanol, strong acid, and NaOH; Autoclavability; Patented sealing gasket to eliminate lateral leakageMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMouse serum (5 μl) was diluted in 200 μl of 1× PBS and was fixed on a nitrocellulose membrane by suction through Bio-Rad Bio-Dot 96-Well Microfiltration equipment. If you need one of these please consider our auction! This is for a dart blot apparatus as shown. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketPkg of 1, 96-well sample template, modular unit converts Bio-Dot SF apparatus to Bio-Dot apparatus; for blot transfer microfiltrationBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. 11. Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketMicrofiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketMicrofiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasketPkg of 1, 96-well sample template, modular unit converts Bio-Dot SF apparatus to Bio-Dot apparatus; for blot transfer microfiltrationOverview. Bio-Dot microfiltration system. Screen and filter antibodies and sera. Leak-free, modular, and autoclavable template. Browse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. Leak-free, modular, and autoclavable template. Sequi-Blot PVDF membranes (Bio-Rad) were cut to size, wet with MeOH and equilibrated in TE buffer, then assembled into a 96-well Bio-Dot microfiltration apparatus (Bio-Rad). The material used in the construction of the Bio-Dot blotting apparatus. 75 mm. Features of the Bio-Dot Microfiltration System: Resistance to 100% ethanol, strong acid, and NaOH; Autoclavability; Patented sealing gasket to eliminate lateral leakageBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasketBrowse Bio-Rad's microfiltration and screening systems for easy capture of proteins or nucleic acids onto membranes. 2. Leak-free, modular, and autoclavable template. Reproducibly capture proteins or nucleic acids in solution onto membranes with Bio-Dot microfiltration systems.